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There is no validated, publicly available reconstitution protocol for individuals to follow safely

Older people usually produce less stomach acid and therefore less intrinsic factor, increasing their risk for deficiency

Without changing any parameters except for the dose, the human model was validated against four separate datasets (see section 4.3.2) with different PO doses 250mg dose dataset from Chung [36] with one 375mg pre-dose 12 hours before the main dose, 500mg dose dataset from Gusler [37] without a pre-dose, 750mg dose dataset from Wen [38] with one 500mg pre-dose 12 hours before the main dose and a 500mg multiple dose dataset from El Messaoudi [39] with six pre-doses of 500 mg with a time interval of 12h

2019 Jul 12;39(8):NP343NP351