The following secondary antibodies were used: Alexa Fluor 555-labelled donkey anti-rabbit IgG (H+L) (#A0453, Beyotime, Shanghai, China, 1:200), horseradish peroxidase (HRP)-labelled goat anti-rabbit IgG (H+L) (#GB23303-1, Servicebio, 1:8000), HRP-labelled goat anti-mouse IgG (H+L) (#GB23301, Servicebio, 1:8000), and HRP-conjugated goat anti-rabbit IgG (#D110,058, Sangon Biotech, 1:8000)
Vitamin B12 (Methylcobalamin) is better absorbed than Cyanocobalamin and does not contain minute amounts of cyanide
Avoid alcohol, caffeine in excess, and tobacco products, all of which dry out oral tissues further
As a methyl donor, SAM participates in various methyl transfer reactions and is converted to S-adenosylhomocysteine (SAH) in the reaction