10mg tirzepatide vial (lyophilized powder) Bacteriostatic water (not sterile water, not saline, not normal water) Sterile syringe for reconstitution (a 3ml syringe with a needle works well) Alcohol swabs (70% isopropyl alcohol pads) U-100 insulin syringes for dosing (29-31 gauge) Sharps container for disposal Clean, well-lit workspace A note on the water: you must use bacteriostatic water , not sterile water for injection
In addition to a medium made of 0.5 ml of DMEM, 10% FBS, 100 U/mL of penicillin, and 100 mg/mL of streptomycin, BPC 157 was added to each well in concentrations of 0 (control group), 0.1, 0.25, and 0.5 ug/mL for 1, 2, and 3 days in a humidified atmosphere
Of those 36, 35 were preclinical studies conducted in animals
Absence of a strong signal is not the same as a well-characterised safety profile